This article introduces the Layer Analysis Method of the Yellow Emperor's Inner Classic text to revive its use in modern acupuncture and medicine. This is a crucial concept, especially for the diagnosis and treatment of diseases by acupuncture. First, the rise and decline of this method is explored. Second, the differentiation of this method is described by symptoms, the affected organs, and the stage of the disease. Third, the treatment method is summarized into four categories: (1) equipment, (2) technique, (3) acupoint, and (4) pathology.